Evaluation of Chitin as a Biomarker of Pathogenic Fungal Isolates

Chitin is a polysaccharide component of the inner cell wall fungi that has been used to estimate fungal invasion in plant products. However, its detection major pathogenic species not investigated. The present study aimed determine chitin contents order evaluate diagnostic potential as biomarker for infections. High performance liquid chromatography (HPLC) was measure content. Pure acid hydrolyzed and fluorescence 9-fluorenylmethylchloroformate (FMOC-CI) derivatives glucosamine produced were measured. ten isolates determined per mycelial dry weight. They varied from 18.61 (± 0.09) 47.12 0.50) μg/mg Candida albicans Cryptococcus neoformans exhibited highest lowest levels chitin, respectively. Based upon relative amounts produced, three groups namely: high (Candida albicans, gattii, Aspergillus niger Penicilliumat 47.12, 46.98, 46.05, 44.15 respectively), medium (Rhizopus, fumigatus, Fusarium solani, andMucor at 36.61, 36.30, 35.03, 34.84 μg/mg, low tropicalis 20.78 identified. detectable bacterial controls. method offers sensitive specific tool quantification isolates. may be useful assay diagnosis infections clinical samples.